This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000 - 1:35,000, IF Microscopy: User Optimized, WB: 1:300 to 1:500
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in
Western blot using Rocklands affinity purified anti-Nedd4 antibody shows detection of a 115 kDa band (arrowhead) corresponding to endogenous Nedd4. Lane 1: MDA-MB-435S cell lysates (p/n W09-001-A39). The blot was blocked with 5% BLOTTO (p/n B501-0500) overnight at 4C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20,000 dilution of HRP goat anti-rabbit IgG in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). ECL was used for detection.
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