This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-pRb2/p130 antibody, rabbit anti-pRb2 antibody, rabbit anti-p130 antibody, rabbit anti-Spa310 antibody, 130 kDa retinoblastoma associated protein antibody, RBR-2 antibody, RBL2 antibody, RB2 antibody, Retinoblastoma like 2 antibody, Retinoblastoma like protein 2 antibody, Retinoblastoma Related Gene antibody
This affinity purified antibody is tested for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to pRb2/p130, and a band approximately 4.2kDa in s
Western blot using Rocklands affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4 C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4 C. ECL was used for detection. Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.
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