This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-ATDC Ac-K116 Antibody, rabbit anti-ATDC acetylated Lys116 Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:100,000, WB: 1:500 to 3,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user. Expect a band approximately ~66 kDa in size corresponding to ATDC
Western blot using Rocklands affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation. Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.
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