Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:3,000, IHC: User Optimized, IF Microscopy: User Optimized, IP: User Optimized, WB: 1 µg/mL
Application Notes:
RRM2B/p53R2 antibody is tested in Western Blot and ELISA and suitable for IP, IHC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RRM2B1 by western blotting in t
Western blot using Rocklands affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T transfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1µg/mL and incubated overnight at 4C. ECL detection was used.
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