Anti-GAS antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human GAS.
Conjugation:
Unconjugated
Alternative Names:
GAS Antibody, GAS, PA1, C16orf53, PAXIP1-associated glutamate-rich protein 1, PAXIP1-associated protein 1, PAGR1, Glutamate-rich coactivator interacting with SRC1, PTIP-associated protein 1
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-GAS Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 28 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of GAS antibody. Lane 1: EL4 cell lysate with GAS antibody at 1 µg/mL in the absence of blocking peptide. Lane 2: EL4 cell lysate with GAS antibody at 1 µg/mL in the presence of blocking peptide. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 28 kDa, 36 kDa for GAS. Other band(s): GAS splice variants and isoforms.
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