Anti-GJB2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the internal region of human GJB2.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-GJB2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 26 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Rabbit anti-GJB2 antibody. Lane A: human colon tissue lysate at 1 µg/mL. Lane B: human colon tissue lysate at 2 µg/mL. Primary antibody: GJB2 antibody overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 25 kDa, 26 kDa for GJB2.
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