Anti-GPR15 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminus of human GPR15. The sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid (2).
Conjugation:
Unconjugated
Alternative Names:
GPR15 Antibody, BoB, Brother of Bonzo, G-protein coupled receptor 15
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5000 - 1:10000, WB: 0.5-1 µg/mL
Application Notes:
Anti-GPR15 Antibody has been tested for use in ELISA, immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 41 kDa in Western Blots of specific cell lysates and t
Western Blot of GPR15 antibody. Lane 1: Human spleen lysate with GPR15 at 0.5 µg/mL. Lane 2: Human spleen lysate with GPR15 at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 40.8 kDa, 50 kDa for GPR15. Other band(s): GPR15 splice variants and isoforms.
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