Anti-Integrin alpha 4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 13 amino acid synthetic peptide from near the C-terminus of human Integrin alpha 4.
Conjugation:
Unconjugated
Alternative Names:
Integrin alpha 4 Antibody, IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-Integrin alpha 4 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 115 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Integrin alpha 4 antibody. Lane A: Jurkat cell lysate at 1 µg/mL in the absence of blocking peptide. Lane B: Jurkat cell lysate at 1 µg/mL in the presence of blocking peptide. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 114.9 kDa, ~200 kDa for Integrin alpha 4.
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