Anti-IRE1p antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the internal region of human IRE1p.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-IRE1p Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 110 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of IRE1p antibody in Raji cell lysate. Lane A: IRE1p antibody at 1 µg/mL. Lane B: IRE1p antibody at 2 µg/mL. Load: 35 µg per lane. Primary antibody: IRE1p antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 110 kDa, 115 kDa for IRE1p. Other band(s): IRE1p splice variants and isoforms.
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