ARF-BP1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ARF-BP1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-C03
• Supplier Catalog Number: 600-401-C03
• Alternative Catalog Number: ROC-600-401-C03
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: ARF-BP1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ARF-BP1 protein.
• Conjugation: Unconjugated
• Alternative Names: ARF-BP1, ARF binding protein 1, HECT, UBA and WWE domain containing 1, HUWE1, Mcl-1 ubiquitin ligase E3, Mule, E3 ubiquitin-protein ligase HUWE1, KIAA0312, KIAA1578, UREB1

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 10075
• UniProt: Q7Z6Z7
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:40,000, IF Microscopy: 20 µg/mL, WB: 1.0 -2.0 µg/mL
• Application Notes: ARF-BP1 antibody has been tested in ELISA, WB, IF, and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~481 kDa in size corresponding to ARF-BP1 by Western blotting in the appropriate cell lysate or extract.

Images

Immunocytochemistry demonstrating detection of ARF-BP1 in Daudi cells. The primary antibody was used at 5 µg/ml.

Western Blot of ARF-BP1 antibody. Lane 1: Daudi cell lysate at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 481 kDa, ~480 kDa for ARF-BP1. Other band(s): ARF-BP1 splice variants and isoforms.

Immunofluorescence Microscopy of ARF-BP1 antibody. Tissue: Daudi cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: ARF-BP1 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ARF-BP1 is nuclear and occasionally cytoplasmic. Staining: ARF-BP1 as a red fluorescent signa.

Immunocytochemistry of ARF-BP1 antibody. Tissue: Daudi cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: ARF-BP1 antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ARF-BP1 is nuclear and cytoplasmic. Staining: ARF-BP1 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Western blot showing detection of ARF-BP1 in Daudi cell lysate. Primary antibody was used at 1 µg/ml.