This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000-1:16,000, WB: 1:2,500
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphoryl
Western Blot of Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 µg per lane. Primary antibody: The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: PBS-Tween (0.05%) overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrow): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
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