This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:20,000, WB: 2µg/mL
Application Notes:
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell l
Western blot using Rocklands Rabbit anti-SAE1 antibody shows detection of SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody, Anti-SAE1, diluted to 2 µg/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
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