Anti-Dopamine Transporter Antibody was produced in rabbit by repeated immunizations with the synthetic peptide corresponding to the intracellular C-terminal region conjugated to KLH.
Conjugation:
Unconjugated
Alternative Names:
Sodium-dependent dopamine transporter, DA transporter, DAT, Solute carrier family 6 member 3
Clonality:
Polyclonal
Concentration:
Each unit contains titrated antibody for 10 mini-western blots at suggested dilution Sufficient to run approximately 10 miniblots
Anti-Dopamine Transporter Antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 88 kDa corresponding to DAT proteins in the appropriate cell l
Western Blot of Rabbit Anti-Dopamine Transporter C-Terminus Human Antibody. Lane 1: human caudate lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Dopamine Transporter antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~88kDa/~88kDa for DAT protein. Other band(s): none.
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