ERK-MAPK phospho T202/phospho Y204 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ERK-MAPK phospho T202/phospho Y204 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-C76
• Supplier Catalog Number: 600-401-C76
• Alternative Catalog Number: ROC-600-401-C76
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-ERK/MAPK Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Mitogen-activated protein kinase 1, MAP kinase 1, MAPK1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 116590
• UniProt: P63086
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:500, WB: 1:1000
• Application Notes: Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42-44 kDa in size corresponding to ERK/MAPK phosphorylated a

Images

Western Blot of Rabbit anti-ERK-MAPK Thr202-Tyr204 Antibody. Lane 1: human T47D cell lysates. Lane 2: human T47D cell lysates incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: ERK-MAPK Thr202-Tyr204 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~42-44kDa for ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Other band(s): none.