IHC: 1:1000-1:2000, IP: 3µl per 200µg lysate, WB: 1:1000
Application Notes:
Anti-NMDA NR2B subunit (Rabbit) antibody is tested for use in Western Blotting, Immunoprecipitation and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band or approximately 180 kDa in size corresponding to the NR2B
Western Blot of Rabbit anti-NMDA NR2B Subunit antibody. Lane 1: rat hippocampal (Hipp) lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: NMDA NR2B Subunit antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 180 kDa for NMDA NR2B Subunit. Other band(s): None.
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