Anti-p38 MAPK pT180/pY182 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182.
Conjugation:
Unconjugated
Alternative Names:
Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, CRK1, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha
Clonality:
Polyclonal
Concentration:
Titrated value sufficient to run approximately 10 mini blots.
Anti-p38 MAPK pT180/pY182 Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 39kDa in size corresponding to p38 MAPK protein phosphorylated at Thr
Western Blot of Rabbit anti-p38 MAPK Thr180-Tyr182 antibody. Lane 1: HeLa cell lysate untreated (Control). Lane 2: HeLa cell lysate UV treated. Load: 10 µg per lane. Primary antibody: p38 MAPK Thr180-Tyr182 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 39 kDa for p38 MAPK Thr180-Tyr182. Other band(s): none.
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