p38 MAPK phospho T180/phospho Y182 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-C90
Article Name: p38 MAPK phospho T180/phospho Y182 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-C90
Supplier Catalog Number: 600-401-C90
Alternative Catalog Number: ROC-600-401-C90
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human
Immunogen: Anti-p38 MAPK pT180/pY182 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182.
Conjugation: Unconjugated
Alternative Names: Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, CRK1, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha
Clonality: Polyclonal
Concentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 70496
UniProt: P70618
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: IHC: 1:250, WB: 1:1000
Application Notes: Anti-p38 MAPK pT180/pY182 Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 39kDa in size corresponding to p38 MAPK protein phosphorylated at Thr
Western Blot of Rabbit anti-p38 MAPK Thr180-Tyr182 antibody. Lane 1: HeLa cell lysate untreated (Control). Lane 2: HeLa cell lysate UV treated. Load: 10 µg per lane. Primary antibody: p38 MAPK Thr180-Tyr182 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 39 kDa for p38 MAPK Thr180-Tyr182. Other band(s): none.