IHC: 1:2000, IF Microscopy: 1:2000, IP: 1µg per 200µg lysate, WB: 1:1000
Application Notes:
Anti-Synapsin I antibody is tested for Western Blotting, Immunoprecipitation, and IHC and suitable for IF. Expect a band of approximately 78 kDa in size corresponding to the synapsin I doublet in Western Blots of rat brain extracts. Researchers should de
Western Blot of Rabbit anti-Synapsin I antibody. Lane 1: rat hippocampal (Hipp) lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Synapsin I antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 78 kDa for Synapsin I. Other band(s): none.
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