Anti-JPH2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide near the C-terminus of human JPH2.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000, WB: 2 µg/mL
Application Notes:
Anti-JPH2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 74 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of JPH2 antibody. Lane A: 293 cell lysate in the absence of blocking peptide. Lane B: 293 cell lysate in the presence of blocking peptide. Load: 35 µg per lane. Primary Antibody: Anti-JPH2 at 2 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 74.2 kDa, ~74 kDa for JPH2.
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