KLHL1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: KLHL1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-CF1
• Supplier Catalog Number: 600-401-CF1
• Alternative Catalog Number: ROC-600-401-CF1
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Rat
• Immunogen: Anti-KLHL1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the amino-terminus of human KLHL1.
• Conjugation: Unconjugated
• Alternative Names: KLHL1 Antibody, MRP2, KIAA1490, Kelch-like protein 1

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 57626
• UniProt: Q9NR64
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:40,000, IHC: 10 µg/mL, WB: 2-4 µg/mL
• Application Notes: Anti-KLHL1 Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa in Western Blots of specific cell lysates and

Images

Western Blot of KLHL1 antibody. Lane A: human brain cell lysate at 2 µg/ml. Lane B: human brain cell lysate at 4 µg/ml. Load: 35 µg per lane. Primary antibody: KLHL1 antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 83 kDa, 100 kDa for KLHL1 antibody. Other band(s): KLHL1 splice variants and isoforms.