Anti-LGI2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 11 amino acid synthetic peptide from near the N-terminus of human LGI2.
Conjugation:
Unconjugated
Alternative Names:
LGI2 Antibody, KIAA1916, LGIL2, Leucine-rich repeat LGI family member 2, Leucine-rich glioma-inactivated protein 2, LGI1-like protein 2
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-LGI2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 63 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of LGI2 antibody. Lane A: 293 cell lysate at 1 µg/mL. Lane B: 293 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 63 kDa, ~70 kDa for LGI2.
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