5-Lipoxygenase phospho S523 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: 5-Lipoxygenase phospho S523 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-D03
• Supplier Catalog Number: 600-401-D03
• Alternative Catalog Number: ROC-600-401-D03
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Rat
• Immunogen: Anti-5-Lipoxygenase pS523 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523.
• Conjugation: Unconjugated
• Alternative Names: Arachidonate 5-lipoxygenase, 5-LO

Product Properties

• Clonality: Polyclonal
• NCBI: 240
• UniProt: P09917
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-5-Lipoxygenase pS523 antibody has been tested in Western Blotting and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 80 kDa corresponding to the 80 kDa 5-LO protei

Images

Western Blot of Rabbit Anti-5-Lipoxygenase pS523 antibody. Lane 1: rat cortex lysate. Lane 2: rat cortex lysate incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: 5-LO Ser523 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~80k doublet of 5-LO phosphorylated at Ser523. Other band(s): none.