Anti-Dynamin Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
Anti-Dynamin Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to the dynamin proteins in the appropriate cell lys
Western Blot of Anti-Dynamin (Rabbit) Antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Dynamin antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~95kDa/~95kDa for dynamin protein. Other band(s): none.
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