EphrinB phospho Y298 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: EphrinB phospho Y298 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-D35
• Supplier Catalog Number: 600-401-D35
• Alternative Catalog Number: ROC-600-401-D35
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Rat
• Immunogen: Anti-EphrinB pY298 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 of Xenopus EphrinB conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Ephrin type-B receptor 2, EPH-like kinase 5, EK5

Product Properties

• Clonality: Polyclonal
• NCBI: 396513
• UniProt: P28693
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Chicken
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-EphrinB pY298 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB proteins phosphorylated at Tyr298

Images

Western Blot of Anti-EphrinB pT298 (Rabbit) Antibody. Lane 1: rat testes. Lane 2: rat testes incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: EphrinB pT298 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~46kDa/~46kDa for EphrinB phosphorylated at Tyr298. Other band(s): none.