GABA(A) Receptor alpha 1 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-D42
Article Name: GABA(A) Receptor alpha 1 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-D42
Supplier Catalog Number: 600-401-D42
Alternative Catalog Number: ROC-600-401-D42
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Mouse, Rat
Immunogen: Anti-GABA(A) Receptor alpha 1 Antibody was produced by repeated immunizations with a fusion protein from the cytoplasmic loop of the alpha 1 subunit of rat GABAA RA1.
Conjugation: Unconjugated
Alternative Names: Gamma-aminobutyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1
Clonality: Polyclonal
Concentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 29705
UniProt: P62813
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000, WB: 1:1000
Application Notes: Anti-GABA(A) Receptor alpha 1 Antibody is tested for use in IHC and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 1 subunit of the GABA(A
Western Blot of Anti-GABA(A) Receptor alpha 1 (Rabbit) Antibody. Lane 1: mouse forebrain lysates from wild type. Lane 2: mouse forebrain lysates from alpha1-knockout (alpha1-K/O). Load: 10 µg per lane. Primary antibody: GABA(A) Receptor alpha 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~51kDa/~51kDa for alpha1-subunit of the GABAA-R. Other band(s): none.