Anti-GABA(A) Receptor alpha 1 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region of rat GABAA RA1 conjugated to KLH.
Anti-GABA(A) Receptor alpha 1 (Rabbit) Antibody is tested for use in Western Blot and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 1 subunit of the G
Western Blot of Anti-GABA(A) Receptor alpha 1 (Rabbit) Antibody. Lane 1: mouse forebrain lysates from Wild Type. Lane 2: mouse forebrain lysates from alpha1-knockout (alpha1-K/O). Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~51kDa/~51kDa for alpha1-subunit of the GABAA-R. Other band(s): none.
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