GABA(A) Receptor alpha 3 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-D46
Article Name: GABA(A) Receptor alpha 3 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-D46
Supplier Catalog Number: 600-401-D46
Alternative Catalog Number: ROC-600-401-D46
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Mouse, Rat
Immunogen: Anti-GABA(A) Receptor alpha 3 Antibody was produced by repeated immunizations with a synthetic peptide from the N-terminal region of the alpha 3 subunit of rat GABAA.
Conjugation: Unconjugated
Alternative Names: Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3
Clonality: Polyclonal
NCBI: 24947
UniProt: P20236
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: IHC: 1:100, WB: 1:1000
Application Notes: Anti-GABA(A) Receptor alpha 3 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 3 subunit of t
Western Blot of Anti-GABA(A) Receptor alpha 3 (Rabbit) Antibody. Lane 1: mouse brain lysates from wild type. Lane 2: mouse brain lysates from alpha3-knockout. Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~51kDa/~51kDa for alpha3-subunit of the GABAA-R. Other band(s): none.