Interferon alpha Receptor Type I phospho S535/phospho S539 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Interferon alpha Receptor Type I phospho S535/phospho S539 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-D67
• Supplier Catalog Number: 600-401-D67
• Alternative Catalog Number: ROC-600-401-D67
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-Interferon alpha Receptor Type I pS535/pS539 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539.
• Conjugation: Unconjugated
• Alternative Names: Interferon alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1

Product Properties

• Clonality: Polyclonal
• NCBI: 3454
• UniProt: P17181
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: IHC: 1:1000, WB: 1:1000
• Application Notes: Anti-Interferon alpha Receptor Type I pS535/pS539 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. The molecular weight of the IFNAR1 varies with cell line due to d

Images

Western Blot of immunoprecipitation of Rabbit anti-Interferon alpha Receptor Type I pS535/pS539 antibody. Lane 1: HEK 293 cells transfected with mock. Lane 2: HEK 293 cells transfected with IFNAR1 WT. Lane 3: HEK 293 cells transfected with IFNAR1 S535A and S539A mutants. Load: 10 µg per lane. Left Panel: control. Center Panel: blocked by the phosphopeptide. Right Panel: dephosphopeptide. Primary antibody: Interferon alpha Receptor Type I pS535/pS539 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~110kDa to ~130kDa for IFNAR1 WT. Other band(s): none.