Anti-PPAPDC1B antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide near the N-terminus of human PPAPDC1B.
Conjugation:
Unconjugated
Alternative Names:
PPAPDC1B Antibody, DPPL1, HTPAP, DPPL1, Phosphatidate phosphatase PPAPDC1B, Phosphatidic acid phosphatase type 2 domain-containing protein 1B
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-PPAPDC1B Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 29 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of PPAPDC1B antibody. Lane 1: EL4 cell lysate with PPAPDC1B antibody at 1 µg/mL. Lane 2: EL4 cell lysate with PPAPDC1B antibody at 2 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 29 kDa, 40 kDa for PPAPDC1B. Other band(s): PPAPDC1B splice variants and isoforms.
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