Anti-PTEN antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the C-terminus of human PTEN.
Conjugation:
Unconjugated
Alternative Names:
PTEN Antibody, BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, IP: User Optimized, WB: 1 µg/mL
Application Notes:
Anti-PTEN Antibody has been tested for use in ELISA, Western Blotting and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 47 kDa in Western Blots of specific cell lysates and tis
Western Blot of PTEN antibody in PC-3 cell lysate. Lane A: PTEN antibody in the absence of blocking peptide. Lane B: PTEN antibody in the presence of blocking peptide. Load: 35 µg per lane. Primary antibody: PTEN antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 47 kDa, 42 kDa for PTEN. Other band(s): PTEN splice variants and isoforms.
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