RHOT1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: RHOT1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-DZ0
• Supplier Catalog Number: 600-401-DZ0
• Alternative Catalog Number: ROC-600-401-DZ0
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-RHOT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the N-terminus of human RHOT1.
• Conjugation: Unconjugated
• Alternative Names: Ras homolog family member T1, mitochondrial Rho GTPase 1, ARHT1, MIRO-1, MIRO1, MIRO

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 55288
• UniProt: Q8IXI2
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
• Application Notes: Anti-RHOT1 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 71 kDa in Western Blots of specif

Images

Western Blot of Rabbit anti-RHOT1 antibody. Lane A: rat brain tissue lysate. Primary antibody: RHOT1 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 76 kDa, 78 kDa for RHOT1.