p53 phospho S392 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: p53 phospho S392 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-E04
• Supplier Catalog Number: 600-401-E04
• Alternative Catalog Number: ROC-600-401-E04
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-p53 pS392 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392.
• Conjugation: Unconjugated
• Alternative Names: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Product Properties

• Clonality: Polyclonal
• Concentration: titrated reagent Sufficient to run approximately 10 miniblots
• NCBI: 7157
• UniProt: P04637
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-p53 pS392 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to p53 protein phosphorylated at Ser392 in the appropriate

Images

Western Blot of Rabbit anti-p53 pS392 antibody. Lane 1: rat brain nuclear fraction lysate (control). Lane 2: lambda-Ptase. Load: 10 µg per lane. Primary antibody: p53 pS392 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 53 kDa for p53 pS392. Other band(s): none.