Anti-Periostin Antibody was produced by repeated immunizations with bacterial fusion proteins equivalent to a 188 amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively spliced exons.
Conjugation:
Unconjugated
Alternative Names:
PN, Osteoblast-specific factor 2, OSF-2
Clonality:
Polyclonal
Concentration:
titrated reagent Sufficient to run approximately 10 miniblots
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
IHC: 1:100, WB: 1:1000
Application Notes:
Anti-Periostin Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 93 kDa in size corresponding to periostin doublet in mouse lung extract. The ant
Western Blot of Rabbit anti-Periostin antibody. Lane 1: rat lung lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Periostin antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 93 kDa Doublet for Periostin. Other band(s): Periostin splice variants and isoforms.
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