Raf-1 phospho S301 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Raf-1 phospho S301 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-E23
• Supplier Catalog Number: 600-401-E23
• Alternative Catalog Number: ROC-600-401-E23
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Rat
• Immunogen: Anti-Raf-1 pS301 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf

Product Properties

• Clonality: Polyclonal
• NCBI: 24703
• UniProt: P11345
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-Raf-1 pS301antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 74 kDa in size corresponding to the Raf-1 protein phosphorylated at Ser301 in the appro

Images

Western Blot of Rabbit anti-Raf-1 pS301 antibody. Lane 1: UV treated human Jurkat cell lysate (Control). Lane 2: lambda-Ptase. Load: 10 µg per lane. Primary antibody: Raf-1 pS301 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 74 kDa for Raf-1 pS301. Other band(s): Raf-1 pS301 splice variants and isoforms.