Anti-RTP801 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the N-terminus of human RTP801.
Conjugation:
Unconjugated
Alternative Names:
RTP801 Antibody, Dig2, REDD1, REDD-1, RTP801, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:20,000-1:40,000, WB: 2-4 µg/mL
Application Notes:
Anti-RTP801 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 25 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of RTP801 antibody. Lane A: 293 cell lysate at 2 µg/mL. Lane B: 293 cell lysate at 4 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 25.3 kDa, ~27 kDa for RTP801.
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