Anti-RUNX1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human RUNX1.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-RUNX1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 49 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of RUNX1 antibody. Lane 1: Raji cell lysate with RUNX1 antibody at 1 µg/mL. Lane 2: Raji cell lysate with RUNX1 antibody at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 49 kDa, 55 kDa for RUNX1. Other band(s): RUNX1 splice variants and isoforms.
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