0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized
Application Notes:
Anti-Seasonal H1N1 Nucleocapsid Protein Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 56 kDa in Western Blots of specific cell lysates and tissues.
ELISA of Seasonal H1N1 Nucleocapsid Protein antibody. Antigen: BSA conjugates of Seasonal H1N1 Nucleocapsid Protein. Coating amount: 60, 10, 2, and 0 ng per well. Primary antibody: Seasonal H1N1 Nucleocapsid Protein antibody at 1 µg/mL. Dilution series: 2-fold. Mid-point concentration: 5 ng/mL Seasonal H1N1 Nucleocapsid Protein antibody. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000. Substrate: TMB (p/n TMBE-0100).
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