SIGLEC15 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: SIGLEC15 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-EK3
• Supplier Catalog Number: 600-401-EK3
• Alternative Catalog Number: ROC-600-401-EK3
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-SIGLEC15 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the N-terminus of human SIGLEC15.
• Conjugation: Unconjugated
• Alternative Names: SIGLEC15 Antibody, CD33L3, HsT1361, SIGLEC-15, CD33L3, Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3, Siglec-15

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 284266
• UniProt: Q6ZMC9
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 2.5 µg/mL, WB: 1-2 µg/mL
• Application Notes: Anti-SIGLEC15 Antibody has been tested for use in ELISA, Western Blotting, and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 36 kDa in Western Blots of specific cell lysates a

Images

Immunofluorescence of SIGLEC15. Tissue: Mouse Spleen. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: SIGLEC15 at 20 µg/mL. Secondary: goat anti-rabbit IgG antibody at 1:500 dilution (red) and DAPI staining (blue).

Western Blot of SIGLEC15. Load: 15 µg of human liver or kidney lysates.Primary Antibody: SIGLEC15 at 2 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence of SIGLEC15. Tissue: Rat Small Intestine. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: SIGLEC15 at 10 µg/mL. Secondary Antibody: goat anti-rabbit IgG antibody at 1:500 dilution (red) and DAPI staining (blue).

Western Blot of SIGLEC15. Load: 15 µg of Mouse Kidney lysate. Primary Antibody: SIGLEC15 at 2 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000

Immunofluorescence of SIGLEC15.Tissue: Human Spleen. Fixation: 4% paraformaldehyde-fixed. Primary Antibody: SIGLEC15 at 10 µg/mL. Secondary: goat anti-rabbit IgG antibody at 1:500 dilution (red) and DAPI staining (blue).

Western Blot of SIGLEC15.Load: 15 µg of Rat Testis lysate.Primary Antibody: SIGLEC15 at 2 µg/mL for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot of SIGLEC15. Load: 15 µg of human kidney lysate. Primary Antibody: SIGLEC15 at (Lane A: 1µg/mL, Lane B: 2µg/mL) for 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.