Anti-SKA2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the N-terminus of human SKA2.
Conjugation:
Unconjugated
Alternative Names:
SKA2 Antibody, FAM33A, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000 - 1:10,000, WB: 0.5-1 µg/mL
Application Notes:
Anti-SKA2 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 14 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of SKA2 antibody. Lane 1: 3T3 cell lysate with SKA2 antibody at 0.5 µg/mL. Lane 2: 3T3 cell lysate with SKA2 antibody at 1 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 14 kDa, 15 kDa for SKA2. Other band(s): SKA2 splice variants and isoforms.
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