Anti-SLFN12 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 12 amino acid synthetic peptide from near the C-terminus human SLFN12.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1 µg/mL
Application Notes:
Anti-SLFN12 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 67 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of SLFN12 antibody. Lane 1: SK-N-SH cell lysate in the absence of blocking peptide. Lane 2: SK-N-SH cell lysate in the presence of blocking peptide. Primary Antibody: Anti-SLFN12 antibody at 1 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 67 kDa, 60 kDa for SLFN12. Other band(s): SLFN12 splice variants and isoforms.
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