Anti-Hemagglutinin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the novel swine influenza Hemagglutinin protein.The peptide sequence is unique from the peptide sequence for product 5237 and 5241.This antibody is a cognate pair with product 5231.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
H1N1 virus
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: User Optimized
Application Notes:
Anti-Swine H1N1 Hemagglutinin Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 63 kDa in Western Blots of specific cell lysates and tiss
Western Blot of Hemaggutinin antibody. Lane A: recombinant seasonal Hemagglutinin with anti-seasonal Hemagglutinin antibody (5231) at 2 µg/mL. Lane B: recombinant seasonal Hemagglutinin with anti-seasonal Hemagglutinin antibody (5233) at 2 µg/mL. Lane C: swine-origin Hemagglutinin with anti-seasonal Hemagglutinin antibody (5231) at 2 µg/mL. Lane D: swine-origin Hemagglutinin with anti-seasonal Hemagglutinin antibody (5233) at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 70 kDa, 72 kDa for Hemaggutinin. Other band(s): Hemaggutinin splice variants and isoforms.
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