Anti-Hemagglutinin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the novel swine influenza Hemagglutinin protein.The peptide sequence is unique from the peptide sequence for product 5237 and 5233.This antibody is a cognate pair with product number 5239.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
H1N1 virus
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized
Application Notes:
Anti-Swine H1N1 Hemagglutinin Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 63 kDa in Western Blots of specific cell lysates and tissues.
ELISA of Swine H1N1 Hemagglutinin antibody. Antigen: BSA conjugates of Swine H1N1 Hemagglutinin. Coating amount: 50, 10, 2, 0 ng per well. Primary antibody: Swine H1N1 Hemagglutinin antibody at 1 µg/mL. Dilution series: 2-fold. Mid-point concentration: 5 ng/mL Swine H1N1 Hemagglutinin antibody. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000. Substrate: TMB (p/n TMBE-0100).
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