0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Rat
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:200, IHC: User Optimized, IP: User Optimized, WB: 0.5 µg/ml
Application Notes:
Anti-Cu/Zn SOD Antibody is tested for use in WB, IP, IF, and IHC. Expect a band approximately ~23kDa (human) and 19kDa (other species) proteins corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS PAGE immunoblots. Specific cond
Western Blot of Rabbit anti-Cu/Zn SOD antibody. Lane 1: human cell line mix. Lane 2: none. Load: 35 µg per lane. Primary antibody: Cu/Zn SOD antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 15.9 kDa, ~19 kDa for Cu/Zn SOD. Other band(s): none.
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