KDEL Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: KDEL Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-F85
• Supplier Catalog Number: 600-401-F85
• Alternative Catalog Number: ROC-600-401-F85
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KDEL Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against KDEL containing synthetic peptide immunogen.
• Conjugation: Unconjugated
• Alternative Names: Lys Asp Glu Leu, K-D-E-L, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention, Kdelr1

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 10945
• UniProt: P24390
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-KDEL Antibody is tested for use in WB, IF, and IHC. Specific conditions for reactivity should be optimized by the end user.

Images

Western Blot of rabbit anti-KDEL antibody. Load: mixed human cell lysates (300ug/gel). Lane 1: PDI control. Lane 2: KDEL control clone. Lane 3: Grp78 control. Lane 4: SPC-109. Lane 5: Calrecticulin control. Lane 6: KDEL control clone. Lane 7: Grp94: control. Primary antibody: anti-KDEL at 1:500 for overnight at 4C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4C. Predicted/Observed size: 59kDa/45-110 kDa. Other band(s): Recognizes KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.