Anti-TSLP receptor antibody was prepared from whole rabbit serum produced by repeated immunizations with a 21 amino acid synthetic peptide from near the internal region of mouse TSLP receptor.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, IF Microscopy: 20 µg/mL, WB: 1 µg/mL
Application Notes:
Anti-TSLP Receptor Antibody has been tested for use in ELISA, Western Blotting and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 38 kDa in Western Blots of specific cell lysates
Western Blot of TSLP Receptor antibody. Lane A: Mouse heart tissue lysate in the presence of blocking peptide. Lane B: Mouse heart tissue lysate in the absence of blocking peptide. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 117.9 kDa, ~50 kDa for TSLP Receptor antibody. Other band(s): TSLP Receptor antibody splice variants and isoforms.
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