XAF-1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: XAF-1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-FZ6
• Supplier Catalog Number: 600-401-FZ6
• Alternative Catalog Number: ROC-600-401-FZ6
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Anti-XAF-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
• Conjugation: Unconjugated
• Alternative Names: XAF-1 Antibody, BIRC4BP, XIAPAF1, HSXIAPAF1, BIRC4BP, XIAP-associated factor 1, BIRC4-binding protein

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 54739
• UniProt: Q6GPH4
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5000 - 1:20,000, IHC: 2 µg/mL, IF Microscopy: 10 µg/mL, WB: 0.5-2 µg/mL
• Application Notes: Anti-XAF-1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 35 kDa in Western Blots of specifi

Images

Immunofluorescence Microscopy of XAF-1 antibody. Tissue: human spleen cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: XAF-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: XAF-1 is located in the cytoplasm, cytoplasmic vesicle, cytoskeleton, endosome, golgi apparatus, and cell membrane. Staining: XAF-1 as red fluorescent signal.