ERK2 C-Term Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ERK2 C-Term Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-GP1
• Supplier Catalog Number: 600-401-GP1
• Alternative Catalog Number: ROC-600-401-GP1
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-ERK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-ERK2 antibody, MAPK1, ERK 2, ERK-2, P42MAPK, PRKM1, PRKM2, MAPK 2, MAP kinase 2, Mitogen-activated protein kinase 2, p42-MAPK, MAP kinase isoform p42, Extracellular signal-regulated kinase 2, ERT1, MAP kinase 1, Mitogen-activated protein kinase 1

Product Properties

• Clonality: Polyclonal
• Concentration: 1.03 mg/mL by UV absorbance at 280 nm
• NCBI: 28482
• UniProt: P28482
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000, WB: 1µg/mL
• Application Notes: Anti-ERK 2 (RABBIT) antibody has been tested in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.

Images

Western Blot of Rabbit anti-ERK2 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lysate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 10 µg per lane. Primary antibody: Anti-ERK2 C-Term Antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 44kDa for ERK2.