Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 5 µg/ml, WB: 1 ug/mL
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 61 kDa in size corresponding to receptor ROR gamma by western blotting in the appro
Western Blot of Rabbit anti-receptor ROR gamma pS203 antibody. Lane 1: Opal PreStained Ladder (p/n MB-210-0500). Lane 2: Wild-type Mouse Thymus. Lane 3: KO Mouse Thymus. Load: 10 µg per lane. Primary antibody: Receptor ROR gamma pS203 antibody at 1 ug/mL for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 minutes at RT. Predicted/Observed size: 63 kDa for ROR gamma.
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