Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.
Conjugation:
Unconjugated
Alternative Names:
Rabbit Anti-Orf2 Antibody, Endonuclease reverse transcriptase, LINE-1 retrotransposable element ORF2 protein, Line1 protein, Orf2p, retrovirus-related Pol polyprotein LINE-1, Long interspersed element-1, L1, Orf-2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1.0 ug/ul, WB: 1.0 ug/ml
Application Notes:
This affinity purified antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~150 kDa in size corresponding to Orf2 by western blotting in the liver cell lysates
Western Blot of Rabbit Anti-Orf2 antibody. Lane 1: Mouse liver whole cell lysate (W10-000-T020). Load: 10 µg per lane. Primary antibody: Orf2 antibody at 1.0 µg/mL for overnight at 4C. Secondary antibody: Gt-a-Rb HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 150 kDa for Orf2.
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