Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
Application Notes:
Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cel
Western Blot of Rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: NIH/3T3 WCL (p/n W10-000-358). Lane 2: NIH/3T3 nuclear extract (p/n W10-001-A74). Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 60 min at RT. Blocking Buffer: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in lane 2 NIH/3T3 nuclear extract (p/n W10-001-A74).
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