Affinity purified Anti-HSF1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the n-terminal portion of human HSF1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 5 µg/ml, WB: 1 ug/ml
Application Notes:
Anti-Heat Shock Factor 1 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of 57 kDa in size corresponding to HSF1 by western blotting in the appropriate cell
Western Blot of Rabbit anti-HSF1 antibody. Lane 1: Ladder Opal PreStained (p/n MB-210-0500). Lane 2: A431 Whole Cell lysate (p/n W09-000-361). Load: 35 µg per lane. Primary antibody: HSF1 antibody at 1.0 ug/ml for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-133) at 1:70,000 for 30 min at RT. Block: Universal Buffer BlockOut (p/n MB-073) for 30 min at room temperature. Predicted/Observed size: ~57kDa for HSF1 in A431 whole cell lysate.
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